| 质粒名称: | DsRed-rab11 WT |
|---|---|
| 出品公司: | Clontech |
| 目录编号: | 12679 |
| 存储实验室: | Richard Pagano |
|
载体骨架基本信息 |
|
| 载体名称: | pDsRed-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4700bp |
| 完整质粒大小: | 5397bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | RAB11A (Homo sapiens)/DsRed-rab11 wild type |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 717bp |
| GenBank编号 | NM_004663 |
| 标签/融合蛋白: |
DsRed (N terminal on backbone)
HA (N terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BAMHI(未破坏) |
| 3’克隆位点: | BAMHI (未破坏) |
| 5’测序引物 | DsRed1-C |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | DsRed-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12679 ; http://n2t.net/addgene:12679 ; RRID:Addgene_12679) |
| 参考文献部分: | Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, Marks DL, Pagano RE. J Clin Invest. 2002 Jun . 109(12):1541-50. 10.1172/JCI15420 PubMed 12070301 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS DsRed-rab11 WT 5397 bp ds-DNA circular SYN 17-JUN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS DsRed-rab11 WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5397)
AUTHORS Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL,
Marks DL, Pagano RE
TITLE Rab proteins mediate Golgi transport of caveola-internalized
glycosphingolipids and correct lipid trafficking in Niemann-Pick C
cells.
JOURNAL J Clin Invest. 2002 Jun . 109(12):1541-50.
PUBMED 12070301
REFERENCE 2 (bases 1 to 5397)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 17, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5397
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 92..395
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 396..599
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 550..570
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 638..647
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 644..1321
/codon_start=1
/product="wild-type DsRed"
/label=DsRed1
/note="mammalian codon-optimized"
/translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
HLFL"
primer_bind complement(827..847)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 1045..1064
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 1239..1262
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 1325..1381
/label=MCS
/note="multiple cloning site"
CDS 1385..1411
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1412..1438
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
polyA_signal 2216..2337
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2253..2272)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2307..2326
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2344..2799)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2481..2502)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2693..2712
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2826..2930
/gene="bla"
/label=AmpR promoter
promoter 2932..3289
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2955..2975)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3140..3275
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3202..3221
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3324..4118
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3378..3397)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3988..4007
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4306..4325)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4350..4397
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4726..5314
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5215..5234
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
601 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtgc gctcctccaa
661 gaacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca
721 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa
781 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
841 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
901 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
961 gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat
1021 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
1081 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct
1141 gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa
1201 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa
1261 cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct
1321 gctcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
1381 catgtaccca tacgatgttc cggattacgc ttacccatac gatgttccgg attacgctgg
1441 tacccgcgac gacgagtacg actacctctt taaagttgtc cttattggag attctggtgt
1501 tggaaagagt aatctcctgt ctcgatttac tcgaaatgag tttaatctgg aaagcaagag
1561 caccattgga gtagagtttg caacaagaag catccaggtt gatggaaaaa caataaaggc
1621 acagatatgg gacacagcag ggcaagagcg atatcgagct ataacatcag catattatcg
1681 tggagctgta ggtgccttat tggtttatga cattgctaaa catctcacat atgaaaatgt
1741 agagcgatgg ctgaaagaac tgagagatca tgctgatagt aacattgtta tcatgcttgt
1801 gggcaataag agtgatctac gtcatctcag ggcagttcct acagatgaag caagagcttt
1861 tgcagaaaag aatggtttgt cattcattga aacttcggcc ctagactcta caaatgtaga
1921 agctgctttt cagacaattt taacagagat ttaccgcatt gtttctcaga agcaaatgtc
1981 agacagacgc gaaaatgaca tgtctccaag caacaatgtg gttcctattc atgttccacc
2041 aaccactgaa aacaagccaa aggtgcagtg ctgtcagaac atctaaggat ccaccggatc
2101 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
2161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
2221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
2281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
2341 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
2401 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
2461 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
2521 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
2581 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
2641 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
2701 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
2761 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
2821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
2881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
2941 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
3001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
3061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
3121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
3181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
3241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
3301 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
3361 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
3421 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
3481 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
3541 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
3601 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
3661 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
3721 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
3781 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
3841 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
3901 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
3961 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
4021 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
4081 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
4141 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
4201 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
4261 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
4321 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
4381 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
4441 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
4501 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
4561 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
4621 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
4681 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
4741 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
4801 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
4861 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
4921 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
4981 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
5041 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
5101 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
5161 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
5221 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
5281 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
5341 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctga
//
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